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Monster caffeine content full#

Use the measuring cylinder to add 25ml of dichloromethane.Invert the separating funnel 3 times, then vent to avoid pressure build-up.

Extract caffeine from the samples take 50ml of the calibration standard or sample and it to a separating funnel.

cola, energy drinks) no preparation is required, skip ahead to step 4 Stir at 500 rpm on a magnetic stirrer for 30 seconds, then leave to cool to room temperature without further stirring.

For tea add 3.2g of dried tea leaves to a 250ml beaker and add 200ml boiling purified water.

For instant coffee add 2g of granules to a 250ml beaker and add 200ml boiling purified water.

These amounts will create 100ppm, 50ppm, 40ppm, 30ppm, 20ppm and 10ppm calibration standards respectively (ppm = mg/L) Make the standards up to the 50ml volume using purified water.

Prepare calibration standards using a pipette add 25ml, 12.5ml, 10ml, 7.5ml, 5ml and 2.5ml to each of 6x 50ml volumetric flasks.

Prepare a standard 1,000 ppm stock solution of caffeine use an analytical balance to weigh 198.2mg of caffeine and make this up to 200ml using purified water in a volumetric flask.

Caffeine (pure) for standard preparation.

UV range Spectrophotometer (with concentration mode preferred).

250ml Conical flasks (one per sample to be tested).

Retort stand with separating funnel ring.

250ml beakers (one per hot drink sample to be tested).

Micropipette – ideally 1ml to 10ml size.

Monster caffeine content full#

The full method below, as originally outlined by the Jenway team at Bibby Scientific, can be used to measure caffeine in drinks for quality control, comparison across different brands or as an educational introduction to working with UV-Vis spectroscopy. The amount of caffeine present in drinks can be measured by UV spectroscopy. A wide variety of drinks contain caffeine – UV spectroscopy allows you to measure the amount present Caffeine is a naturally occurring molecule found in various plants worldwide, and found in beverages such as tea, coffee and soft drinks.